Lukáš Vendl graduated in organ playing from Jan Hora’s class at the Prague Conservatory, and in 2003 from Jaroslav Tůma’s class at the Academy of Performing Arts, Prague. He participated in a number of master classes led by important organists of world renown (such as Martin Sander, Harald Vogel, David Titterington) as well as harpsichordists (Helmut Franke). In 2002, he studied at the Hochschule für Musik und Theater in Hamburg with Wolfgang Zerer. His doctoral thesis deals with the 17th and 18th-century theory of music in relation to the keyboard instrument compositions. He received awards in several competitions in the Czech Republic and abroad.
He works as a Cantor of the Czech Brother Evangelical Church and is the organist of the St. Salvator Church in Prague. He teaches at the Prague Conservatoire and at the Charlotta Masaryk Music School in Prague. His prime interest is the historically informed early music performance.
He carried out several modern premieres of large-scale vocal works, surviving, for example, in the Želiv monastery music collection. He is the leader of the baroque instrument ensemble Harmonia delectabilis and the chamber choir Ensemble Guillaume.
As organist and harpsichordist he gave recitals at the Pilsen Organ Festival, Haydn Festival of Music, Želiv Festival, Budapest Bach Week, Smečno Organ Festival or Orlicko-Kladsko Organ Festival.